|Year : 2020 | Volume
| Issue : 3 | Page : 109-117
Evaluation of the Effects of Lactational Exposure to Hyoscyamine Fraction of Datura stramonium L. Seeds on Learning and Memory in Wistar Rats (Rattus norvegicus)
Idris Tela Abdu1, Sunday Abraham Musa2, Ibrahim Abdullahi Iliya3, James Oliver Nzalak2
1 Anatomy Department, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University, Kano, Nigeria
2 Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
3 Department of Human Anatomy, Federal University Dutse, Jigawa State, Nigeria
|Date of Submission||10-Aug-2020|
|Date of Decision||12-Nov-2020|
|Date of Acceptance||13-Nov-2020|
|Date of Web Publication||25-Dec-2020|
Idris Tela Abdu
Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Science, Bayero University Kano
Source of Support: None, Conflict of Interest: None
Background: Datura stramonium (D. stramonium), is a family member of Solanaceae, an annual plant known for its medicinal and toxic properties.
Aims and Objectives: The study was designed to determine whether lactational exposure to hyoscyamine fraction of Datura stramonium L. (D. stramonium) seeds affect the cognitive, spatial learning and memory functions of the hippocampus in Wistar rats at adulthood.
Materials and Methods: Fresh seeds of D. stramonium L. were procured, macerated and fractionated. Eight (8) Wistar rats weighed 150-250 grams of equal gender were used for the study. The rats were mated and divided into control and treatment groups. Equivalent body weight of normal saline and 400 mg/kgbwt of hyoscyamine fraction were orally administered to the breastfeeding rats respectively on lactational days (LD) 1-21. At adulthood, the rats were subjected to neurobehavioural tests using novel objects recognition (NORT) and Morris water maze (MWM) respectively. The data obtained were expressed as mean ± SEM, independent two samples t-test and General Linear Model (GLM) repeated-measures ANOVA with Fisher's multiple comparisons post-hoc tests were used to show the mean differences using Minitab 17 (LLC., U.K.) statistical package software. P < 0.05 was considered statistically significant.
Results: A significant increase in the meantime of exploration between the groups (p = 0.049) was observed during the NORT. No statistically significant increase (p = 0.626) in the meantime to locate the escape platform between the groups during the MWM test. The CA1 region of the treated group showed mild nuclear hyperchromasia, cytoplasmic vacuolations and pyknosis.
Conclusion: In conclusion, exposure to hyoscyamine fraction of D. stramonium L. seeds at lactation caused neuronal lesions in the CA1 region, impaired novel objects recognition but not spatial learning and memory in Wistar rats at adulthood.
Keywords: D. stramonium, hippocampus, histology, hyoscyamine, lactation
|How to cite this article:|
Abdu IT, Musa SA, Iliya IA, Nzalak JO. Evaluation of the Effects of Lactational Exposure to Hyoscyamine Fraction of Datura stramonium L. Seeds on Learning and Memory in Wistar Rats (Rattus norvegicus). J Neurobehav Sci 2020;7:109-17
|How to cite this URL:|
Abdu IT, Musa SA, Iliya IA, Nzalak JO. Evaluation of the Effects of Lactational Exposure to Hyoscyamine Fraction of Datura stramonium L. Seeds on Learning and Memory in Wistar Rats (Rattus norvegicus). J Neurobehav Sci [serial online] 2020 [cited 2023 Feb 6];7:109-17. Available from: http://www.jnbsjournal.com/text.asp?2020/7/3/109/304918
| Introduction|| |
Breastfeeding has an advantage for both infants and mothers. It provides the nutritional requirement of developing of infants (2014). It gives optimal nutrition and improves mental performance and neurological development. It also enhances immunity, of the developing infants. It decreases the chances of unexpected death of infants, allergic diseases, and development of Type-1 and Type-2 diabetes mellitus,,, when compared to infant formula. Breastfeeding reduces postpartum depression and bleeding and improves weight control. Transfer of medical substances by the breastfeeding women, to their babies is a matter of concern. In humans, the medicines that circulate in the maternal bloodstream can be transferred to their babies through lactation, hence exposing the infants to such medicines which may potentially be harmful., Some of the conventional medicines indicated to have compromised milk production include cabergoline, bromocriptine, ergotamine, pseudoephedrine, and estrogens., Nowadays, the patronage of traditional herbs is on the rise globally. Many developed countries across the globe including the United States,, Canada, the United Kingdom,, United Arab Emirates, and Australia (James and Bah, 2014),,,,,, have reported significant patronage of traditional herbs among the general population. The trend is similar in Africa such that either modern healthcare and medicine is often available only to a limited number of people, or the facilities are too expensive or too few to carter the needs of too many people.
Datura stramonium, a family member of Solanaceae, is an annual plant that possesses phytoconstituents, with alkaloids possessing strong anticholinergic properties.,,, They competitively antagonize acetylcholine (ACh) at peripheral and central muscarinic receptors for the common binding site., Its leaves are used to bust up sagged breast among Pakistan women, while seeds are taken in a cup of green tea to relieve headache.
In Nigeria, the juice of Datura leaves mixed with warm milk is used to expel intestinal worms, while the seeds in palm oils are used for external treatment of insect bites and stings. Both seeds and leaves of Datura are consumed by the youths as a part of the local beverage (Zobo) or in porridge for recreation at ceremonies and public stroke-beating (Shadi/Sharo) in the suitors of Fulani tribe. This marks a sign of courage and responsibility to the suitress and its family. Pieces of literature have reported that D. stramonium decreases the production of breast milk; however, little was reported as to whether it is safe or harmful to the nursing infant if consumed at lactation.
The current study aimed to find out whether maternal ingestion of hyoscyamine fraction of D. stramonium L. seeds at lactation affects the cognitive function of the hippocampus in Wistar rats at adulthood. The study may provide awareness on the risk associated with the ingestion of psychoactive ethnomedicinal plants such as D. stramonium L. seeds during lactation on the memory functions of the hippocampus.
| Material and Methods|| |
The study was approved by the ethics committee of Ahmadu Bello University Committee on Animal Use and Care (ABUCAUC/2018/042) which is in line with the Declaration of Helsinki.
Fresh D. stramonium seeds were procured from Sharada residential area of Kano Municipal Local Government, Kano State, Nigeria. The seeds were identified, and a voucher number (VN108) was issued at the herbarium of the Botany Department, Faculty of Life Sciences, Ahmadu Bello University (ABU), Zaria, Kaduna State, Nigeria. The seeds were separated from the pods, washed thoroughly with clean tap water, and air-dried under shade. Two thousand grams of the dried seeds was weighed using a digital weighing machine and grounded to a pulp using an electronic blender. The powdered sample was collected into a sterile cellophane bag and kept in a cool dry place for extraction.
Ethanol extraction of crude Datura stramonium seeds
Extraction was carried out using cold maceration according to Djilani et al., 2006. Two thousand grams of pulverized seeds was soaked in 1500 mL of 70% (v/v) ethanol at room temperature and allowed to macerate for 72 h. The extract was filtered and the solvent was evaporated in a water-bath at 40°C. The residue was dissolved in 250 mL of H2O, acidified with few drops of H2SO4 to pH 3–4, extracted with petroleum ether and diethyl ether to remove lipophilic, acidic, and neutral material, and basified with the aqueous solution of NH4OH (0.25 M) at pH 9–10. The extract was washed with distilled water to neutral pH, dried with Na2SO4, and concentrated to dryness under reduced pressure to obtain crude alkaloids.
Fractionation of hyoscyamine
The fractionation was carried out according to Salamah and Ningsih, 2017. Five grams of the viscous extract was dissolved in 10 mL of water. The solution was then poured into a separating funnel, added with 10 mL of chloroform, and shaken to solve with two phases, namely water and chloroform. These two phases were separated and collected. This was repeated until the chloroform phase had the same color as the chloroform solvent. The chloroform was then evaporated and recrystallized to obtain the hyoscyamine fraction. The alkaloid was analyzed with ultraviolet (UV)-visible spectrophotometric method. The extraction and fractionation were carried out at the Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, ABU, Zaria.
Quantification of hyoscyamine fraction
High-performance liquid chromatographic condition
A reversed-phase Techsphere 50DS C18 HPLC column (25 cm × 4.6 mm i.d., particle size 5 μm, Supelco, Bellefonte, PA, USA) with oven temperature of 40°C in conjunction with UV adsorption detector operating at 270 nm was employed. The mobile phase was a mixture of 20% acetonitrile, and 45% methanol, 35% water (H2O), and 0.1 mol/L phosphoric acids which adjusted the pH to 7.0 and flow rate of 1 mL/min. A calibration curve for L-hyoscyamine was plotted to determine the amount of the hyoscyamine in the sample fraction. All analyses were carried out at the Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, ABU, Zaria.
Eight healthy Wistar rats are comprised of equal numbers of adult males and virgin females were procured from the Animal House of the Anatomy Department, Faculty of Basic Medical Sciences, Bayero University, Kano, Nigeria. The animals were transported to the Animal House of the Pharmacology Department, Faculty of Pharmaceutical Sciences, ABU, Zaria, Kaduna State, Nigeria. The males were separated from the females and housed and allowed to acclimatize for 2 weeks at ambient temperature, with alternate day and night cycles at the natural condition. Rat chow (Vital feeds®) and tap water were made available to the animal's ad libitum. The median lethal dose (LD50) of hyoscyamine fraction was determined using Lorke's method. Neurotoxicity symptoms were observed, and the animals were allowed for 24 h to be observed for mortality.
Synchronization and phase determination of the estrus cycle and mating
The female rats were injected with an equivalent bodyweight (bwt) of Zoladex® (3.6 mg AstraZeneca) intraperitoneally to synchronize their estrus cycle. Twenty-four hours after synchronization, vaginal smears were collected by vaginal lavage using a 1 mL plastic pipette filled with 10 μL of normal saline (NaCl 0.9%). The tip of the pipette was gently but superficially inserted into the rat vagina. The vaginal fluid was carefully aspirated and placed on a cleaned glass slide. A different glass slide was used for each rat, and unstained material was viewed under a light microscope, without the use of the condenser lens, at ×10 objective lenses. The proportion of the round nucleated cells, cornified cells, and the leukocytes among them were used to determine phases of the estrous cycle.[Figure 1]a
|Figure 1: (a and b) Proestrous phase and sperm tails in vaginal smears of Wistar rats showing nucleated leukocytes and sperm tails 24 hrs after mating (x 10 magnification).|
Click here to view
The animals were randomly selected and divided into two groups; control and treatment. Each group contained a total of eight rats in the ratio of 1:1 adult male to virgin female. Animals in each group were allowed to mate freely, and evidence of mating was established by the presence of sperm tails in the vaginal smears collected and viewed under a light microscope after 24 h [Figure 1]b. Abdominal palpation was carried out to avoid error due to pseudopregnancy. The pregnant dams detected were isolated and transferred to maternity cages. Animal from the same group was kept closely but separately together in different cages. A total of 40 pups were obtained at after parturition. The control and treatment groups received an equivalent bwt of a single dose of normal saline and 400 mg/kg bwt of hyoscyamine fraction of D. stramonium seeds, respectively, orally daily for 3 weeks, from the lactational day (LD) 1 to 21. After treatment, the animals were nurtured to adulthood (PND 75).
Novel object recognition test
This was conducted at adulthood (PND 60–75). The aim was to test short-term memory according to Gaskin et al., 2010. The test consisted of three phases, i.e., habituation, sampling, and test, which was completed in 2 days. For each phase of this test, the open field arena was thoroughly cleaned with an unscented bleach germicidal wipe, 70% ethanol followed by distilled water before initial use. A day before object exposure, the rats were habituated to the open field arena in a 50 cm × 50 cm wooden box. Before the habituation session, a digital video camera (Model DCR-PJ5E, SONY®) was used for proper video coverage of the rats' activity in the maze. A rat at a time was gently removed from the home cage and placed at the center of the arena. The video covering system was turned on and the rat was allowed to freely explore the arena for 10 min. At the end of every session, the arena thoroughly sanitized before the next session began. This was repeated for all the rats until all got habituated the arena. The same setup was maintained during the sampling and test phases. Two identical objects (A1 and A2) and two unidentical objects, one familiar (A) and other, novel (B) were used during the two phases respectively. In both cases, the exploration time allowed was increased to 15 minutes. The object bias score was calculated according to Ennaceur and Delacour.
Morris water maze
This was carried out also at adulthood (PND 60–75) for 6 consecutive days using Morris protocol to test spatial learning and memory. The apparatus consisted of a circular aluminum tank of 100 cm diameter and 60 cm depth with an escape platform of 20 cm long and 12 cm diameter, filled with a pool of clean water to about the two-third depth of the tank at 22–25°C, deep enough to expose 2.54 cm (1 inch) of the platform above the water surface. A digital video device (Model DCR-PJ5E, SONY®) was suspended directly over the pool to capture the entire setup. The rats were trained for 5 days with methylene blue-stained water that submerged the platform 1 inch beneath except for the pretraining day 1, where the platform was 1 inch above the clean and clear water. A latency period of 60 s was allowed for each rat to find the platform. This was repeated for all the rats at five different locations by changing the positions of the platform in the pool within the north, east, south, and west directions following Qing et al. protocol. On day 6, the test day, the setup was maintained as the previous days except that 30 s per trial with no escape platform was observed. The time taken for each rat to identify the usual position of the platform was recorded, and all videos recorded for the trials were analyzed for the escape latency.
Animal sacrifice and histological methodology
The animals were euthanized using 75% ketamine (10 mg/mL USP) anesthesia, and the brains were dissected, removed, and preserved in Bouin's fluid for histological procedures. The tissues were processed in the Department of Pathology, Ahmadu Bello University Teaching Hospital, Shika, Zaria, Kaduna State, Nigeria. The brain tissues were dehydrated (graded alcohol) and cleared (in xylene) using an automatic processing machine (Shandon Southern Duplex Processor). The tissues were embedded in paraffin wax and blocked in the coronal plane. Serial sections of the blocks were taken at 8 μm with a Leitz Wetzlar microtome, mounted on glass slides, and allowed to dry overnight. The staining technique employed was hematoxylin and eosin (H and E) in paraffin sections. Sections were viewed under a light Olympus Binocular Microscope (CH20i, Uttar Pradesh, India) at high magnifications (×40) and micrographs were taken with the help of Celestron® eyepiece digital camera (EC 3.0MP, China). The sections of the hippocampus were observed in the treated rats and compared to the controls.
The data obtained were expressed as mean ± standard error of the mean; independent sample t-test and pairwise general linear model repeated-measures ANOVA followed with Fisher's multiple comparisons' post hoc test were carried out to find the mean differences in the escape latency, exploration, discrimination, and novelty preference time between groups using Minitab 17 (LLC, United Kingdom Inc. www.minitab.com) GraphPad Software. A P < 0.05 was considered statistically significant. All figures and charts were constructed using (San Diego, California USA, www.graphpad.com).
| Results|| |
No mortality was observed in the first phase when the animals received 10, 100, and 1000 mg/kg bwt. However, toxicity symptoms characterized by restlessness (hyperactivity), labored breathing, piloerection, abdominal cramps, stooling (diarrhea), and urination were observed especially at 1000 mg/kg bwt. The symptoms later disappeared and the animals became calm, weak, and quiet. During the second phase, the symptoms persisted with high intensity in all the groups treated with 1600, 2900 and 5000 mg/kg bwt. Neurotoxicity symptoms were observed, but no mortality was recorded even at the highest dose. The D. stramonium fraction was therefore considered safe in Wistar rats and 5000 mg/kg bwt was taken as the LD50.
[Figure 2] shows the percentage exploration time in the sampling phase of cognitive function test using novel object recognition test (NORT) using two identical objects (A1 and A2) between the controls and adult Wistar rats treated with 400 mg/kg bwt hyoscyamine-treated groups. There was no statistically significant difference (P > 0.05) in the exploration time for object exploration. Any rat that scored less than or above 20% or 80%, respectively, was excluded for the test phase of the experiment.
|Figure 2: Sampling phase of Novel object recognition in Wistar rats exposed to an equivalent bodyweight of normal saline and 400 mg/kgbwt hyoscyamine fraction of D. stramonium seeds at lactational day (LD)1-21.|
Click here to view
The result of comparisons of the NORT in the test phase between the control and treated groups is shown in [Figure 3]. The time spent to explore the novel object (B) decreases significantly (P = 0.049) compared to the familiar (A). Although there was an increase in the time taken to explore novel object (B) in the treated group, there was not statistically significant (P = 0.238).
|Figure 3: Test-phase in the novel object recognition test in Wistar rats exposed to an equivalent bodyweight of normal saline and 400 mg/kgbwt hyoscyamine fraction of D. stramonium seeds at lactational day (LD)1-21. *p = 0.049.|
Click here to view
[Figure 4] shows the comparison of discrimination index in novel object between the control and treated groups. No statistically significant difference (P > 0.05) in the discrimination ability between the groups was observed.
|Figure 4: Discrimination index of novel object recognition test in Wistar rats exposed to an equivalent bodyweight of normal saline and 400 mg/kgbwt hyoscyamine fraction of D. stramonium seeds at lactational day (LD)1-21.|
Click here to view
[Figure 5] shows a comparison of novelty preference between the groups. The time taken to explore the novel object between the groups was not statistically significant (P = 0.411). Thus, the control and the treated groups had an equal preference for the novel object.
|Figure 5: Novelty preference in Wistar rats exposed to an equivalent bodyweight of normal saline and 400 mg/kgbwt hyoscyamine fraction of D. stramonium seeds at lactational day (LD)1-21.|
Click here to view
[Figure 6] shows a comparison of the spatial learning and memory test. No significant differences in the time taken to locate the escape platform between groups (F (1, 54) = 0.02, P = 0.875) or between groups and days (F (5, 54) = 0.64, P = 0.670) were observed. Similar observations were made in the control group on the 2nd and 5th training days (P > 0.05). On the 4th training day, the two groups located the escape platform at an equal time with no statistically significant (P = 0.799) difference in the escape latencies. On the 6th day (probe), the escape latency time was shorter in the treated group compared to the control group but not significant statistically (P = 0.626).
|Figure 6: Morris water maze test in Wistar rats exposed to an equivalent bodyweight of normal saline and 400 mg/kgbwt hyoscyamine fraction of D. stramonium seeds at lactational day (LD)1-21. p = 0.626.|
Click here to view
[Figure 7] a and [Figure 7]b shows H and E-stained photomicrographs of cornu ammonis 1 (CA1) regions in adult Wistar rats at 12th week after being treated with normal saline and hyoscyamine fraction of D. stramonium L. seeds from the LD 1 to 21 as control and treated groups, respectively. The control group showed that normal histology of the SO, SR, and PCL with slight patches of oligodendrocytes as compared to the treated group was slight patches of smaller but mild nuclear hyperchromasia nuclei (black arrows), mild cytoplasmic vacuolations (black asterisk), and mild pyknotic cells (white arrowhead).
|Figure 7: (a) Cornu Ammonis (CA1) region of the hippocampus in Wistar rats exposed to an equivalent body weight of normal saline at lactational day (LD)1-21. (H&E, x 400). PCL = Pyramidal Cell Layer, SO = stratum oriens, SR = stratum radiatum, = oligodendrocytes. (b) Cornu Ammonis (CA1) region of hippocampus in Wistar rats exposed 400 mg/kgbwt hyoscyamine fraction of D. stramonium seeds at lactational day (LD)1-21. (H&E, x 400). PCL = Pyramidal Cell Layer, SO = stratum oriens, SR = stratum radiatum, = pyknotic pyramidal cells, = hyperchromatic granular cells = oligodendrocytes, = cytoplasmic vacuolation.|
Click here to view
| Discussion|| |
Ingestion of hyoscyamine fraction resulted in both central and peripheral neurotoxicity symptoms in the rats fed with graded doses of the fraction. However, no mortality was recorded as a result of ingestion both during toxicity testing and experiment itself. Our literature search was unable to find a related study which determined the median toxicity dose of hyoscyamine fraction of D. stramonium seeds in Wistar rats. We, therefore, propose this research to provide baseline datum for the median toxicity dose of hyoscyamine fraction of D. stramonium seeds in Wistar rats. However, no published literature works are available to make a comparison of the current study in Wistar rats. Considering the foregoing, it could be assumed that oral ingestion of D. stramonium seeds might have a high safety margin in Wistar rats. The clinical symptoms observed might probably result from the anticholinergic properties of tropane alkaloids, which compete and irreversibly inhibit ACh on muscarinic receptors, thereby causing both central and peripheral nervous system manifestations. The central nervous system features include restlessness (hyperactivity), labored breathing, and delirium, while the peripheral symptoms observed include breathing, piloerection, abdominal cramps, stooling (diarrhea), and urination. Similar observations were reported in patients involved in D. stramonium poisoning.
Hyoscyamine accounts for 66% of the total tropane alkaloid content with about 99% of the analyzed Datura seeds as (−)-hyoscyamine. The antimuscarinic activity of hyoscyamine is stereospecifically caused by the (−)-hyoscyamine enantiomer which was estimated to be more potent than the (+)-enantiomer. ACh is one among the main neurotransmitters that cause changes in the brain memory function. It has numerous receptors that are found in various tissues involved in learning and memory. Hyoscyamine competes for ACh by binding to muscarinic receptors of the nervous systems. The ability of tropane alkaloids to cause a change in neurogenesis has been reported with unclear mechanisms of action., The current study did not observe a statistically significant increase in the NORT during the novel object discrimination or novelty preference between the groups, except for the exploration time where the significant increase was observed in the control group, thus more curiosity toward the novel object. There was also no statistically increase in the time taken by the treated rats to locate the escape platform during spatial learning and memory test of the Morris water maze. To the best our knowledge, this is probably the first report that evaluates the effects of exposure to hyoscyamine fraction of D. stramonium seeds on Wistar rats treated at the lactation stage, for neurobehavioral impairments. The lack of cognitive deficits observed in the adulthood perhaps probably indicates that hippocampus exposure to hyoscyamine fraction of D. stramonium seeds does not affect the memory functions of the hippocampus at adulthood in Wistar rats. In a similar study, however, adolescent Wistar rats exposed to atropine were reported to suffer a deficit in hippocampus cognitive function at adulthood. This discrepancy might be attributed to the route of exposure and enantiomerization during the extraction processes, as enantiomerization of hyoscyamine from (−) to (+)-hyoscyamine is possible under aqueous alkaline solution and elevated temperature conditions. Although it was reported that insignificant quantity is obtainable in breast milk, the stage of development of the Wistar rats may also be considered as the possible reason of the discrepancy, as adolescence is considered as a critical period of neuronal plasticity, hence easily susceptible to neurotoxic tendencies. Studies have also found that exposure to tropane alkaloids to influence the quality (unpleasant taste) and quantity (yield) of milk from lactating animals and that passage of substances through breast milk of the lactating mothers to their newborn babies rely on different various factors, which include physical and chemical properties of the substances, maternal physiology, and molecular velocity of substances. By extension, the pups probably did not receive enough dose of the hyoscyamine fraction to cause damage in the hippocampal neurogenesis that could alter the memory. Another reason attributable to this might probably cause by the short exposure duration and rapid metabolization of the alkaloids in the biological system, as it was reported that oral absorption of the anticholinergic agents, such as atropine and glycopyrrolate, was poor as a trace or no amount was reported to be found in breast milk.
Hippocampus locates under the cerebral cortex and plays an important role in memory formation and navigation. It subdivides into narrow areas with distinguished parts, known as CA areas. The cornu ammonis (CA1) neurons plays a vital role in processing memory in the rats. The current study did not observe serious changes in the histology of CA1 regions of the hippocampus between the groups. However, a slight patch of smaller but hyperchromic nuclei, mild cytoplasmic vacuolations, and pyknotic cells were observed in the treated group. These observations indicate changes in the histology of the CA1 region caused by the fraction, probably due to the release of generated neurotoxic elements such as reactive oxygen species resulted from the action of the fraction. Studies have confirmed that release of nitric oxide, p53, residual oxygen species, and cytokines causes excitotoxicity, which could lead to cells loss in the hippocampus.,,, To our knowledge, no similar works of literature offer data about the histopathological effects of hyoscyamine fraction of D. stramonium seeds on pups via lactation; however, Ekanem et al. reported cytoplasmic vacuolation and cellular necrosis in adult Wistar rats treated with ethanol extract of D. stramonium seeds intraperitoneally. Furthermore, Bihaqi et al. reported a neuronal lesion characterized by necrosis, ghost cells, hemorrhage and cytoplasmic vacuolations in rats that received intraperitoneal treatment of scopolamine. All tropane alkaloids of D. stramonium Linn species parts have central anticholinergic symptoms as it can cross the blood–brain barrier and cause long-lasting effects. It induces hypnosis and neuronal degeneration.
| Conclusion|| |
Exposure of Wistar pups to hyoscyamine fraction of D. stramonium L. seeds at lactation causes mild changes in the histoarchitecture of the CA1 region, impaired novel objects recognition ability but not spatial memory in Wistar rats at adulthood.
Patient informed consent
There is no need for patient informed consent.
Ethics committee approval
The ethics committee approval has been obtained from the Ahmadu Bello University Committee on Animal Use and Care (ABUCAUC/2018/042).
Financial support and sponsorship
No funding was received.
Conflicts of interest
There are no conflicts of interest to declare.
Author contribution area and rate
Idris Abdu Tela (50%): Design the research, data collection and analyses and wrote the whole manuscript. Sunday Abraham Musa (20%): Organized the research and supervised the article write-up. Ibrahim Abdullahi Iliya (15%): Contributed with comments on research design and slides interpretation. James Oliver Nzalak (15%): Contributed with comments on manuscript organization and write-up.
| References|| |
Onyiapat JL, Okoronkwo IL, Ogbonnaya NP. Complementary and alternative medicine use among adults in Enugu, Nigeria. BMC Complement Altern Med 2011;11:19. doi: 10.1186/1472-6882-11-19.
Okoronkwo I, Onyia-Pat JL, Okpala P, Agbo MA, Ndu A. Patterns of complementary and alternative medicine use, perceived benefits, and adverse effects among adult users in Enugu Urban, Southeast Nigeria. Evid Based Complement Alternat Med 2014;2014:239372. doi: 10.1155/2014/239372.
Bodeker G, Kronenberg F. A public health agenda for traditional, complementary, and alternative medicine. Am J Public Health 2002;92:1582-91. doi: 10.2105/ajph.92.10.1582.
Zhang Y, Leach MJ, Hall H, Sundberg T, Ward L, Sibbritt D, et al
. Differences between male and female consumers of complementary and alternative medicine in a National US Population: A secondary analysis of 2012 NIHS Data. Evid Based Complement Alternat Med 2015;2015:413173. doi: 10.1155/2015/413173.
Achigbu E, Achigbu K. Traditional medication uses among out-patients attending the eye clinic of a secondary health facility in Owerri, South-East Nigeria. Orient J Med 2014;26:107-13.
Alwhaibi M, Sambamoorthi U. Sex differences in the use of complementary and alternative medicine among adults with multiple chronic conditions. Evid Based Complement Alternat Med 2016;2016:2067095. doi: 10.1155/2016/2067095.
Kaadaaga HF, Ajeani J, Ononge S, Alele PE, Nakasujja N, Manabe YC, et al.
Prevalence and factors associated with use of herbal medicine among women attending an infertility clinic in Uganda. BMC Complement Altern Med 2014;14:27. doi: 10.1186/1472-6882-14-27.
Tsai HH, Lin HW, Simon Pickard A, Tsai HY, Mahady GB. Evaluation of documented drug interactions and contraindications associated with herbs and dietary supplements: A systematic literature review. Int J Clin Pract 2012;66:1056-78. doi: 10.1111/j.1742-1241.2012.03008.x.
Chien LC, Yeh CY, Lee HC, Jasmine Chao H, Shieh MJ, Han BC. Effect of the mother's consumption of traditional Chinese herbs on estimated infant daily intake of lead from breast milk. Sci Total Environ 2006;354:120-6. doi: 10.1016/j.scitotenv.2005.01.033.
Ebrahim AM, Eltayeb MH, Khalid H, Mohamed H, Abdalla W, Grill P, et al
. Study on selected trace elements and heavy metals in some popular medicinal plants from Sudan. J Nat Med 2012;66:671-9. doi: 10.1007/s11418-012-0630-6.
Gatti L. Maternal perceptions of insufficient milk supply in breastfeeding. J Nurs Scholarsh 2008;40:355-63. doi: 10.1111/j.1547-5069.2008.00234.x.
Jackson PC. Complementary and alternative methods of increasing breast milk supply for lactating mothers of infants in the NICU. Neonatal Netw 2010;29:225-30. doi: 10.1891/0730-0818.104.22.168.
Sibeko L, Dhansay MA, Charlton KE, Johns T, Gray-Donald K. Beliefs, attitudes, and practices of breastfeeding mothers from a periurban community in South Africa. J Hum Lact 2005;21:31-8. doi: 10.1177/0890334404272388.
Kimani-Murage EW, Wekesah F, Wanjohi M, Kyobutungi C, Ezeh AC, Musoke RN, et al
. Factors affecting actualisation of the WHO breastfeeding recommendations in urban poor settings in Kenya. Matern Child Nutr 2015;11:314-32. doi: 10.1111/mcn.12161.
Ranasinghe S, Ansumana R, Lamin JM, Bockarie AS, Bangura U, Buanie JA, et al
. Herbs and herbal combinations used to treat suspected malaria in Bo, Sierra Leone. J Ethnopharmacol 2015;166:200-4. doi: 10.1016/j.jep.2015.03.028.
Diaz T, George AS, Rao SR, Bangura PS, Baimba JB, McMahon SA, et al
. Healthcare seeking for diarrhoea, malaria and pneumonia among children in four poor rural districts in Sierra Leone in the context of free health care: Results of a cross-sectional survey. BMC Public Health 2013;13:157. doi: 10.1186/1471-2458-13-157.
James PB, Bah AJ, Tommy MS, Wardle J, Steel A. Herbal medicines use during pregnancy in Sierra Leone: An exploratory cross-sectional study. Women Birth 2018;31:e302-9. doi: 10.1016/j.wombi.2017.12.006.
James PB, Kamara H, Bah AJ, Steel A, Wardle J. Herbal medicine use among hypertensive patients attending public and private health facilities in Freetown Sierra Leone. Complement Ther Clin Pract 2018;31:7-15. doi: 10.1016/j.ctcp.2018.01.001.
Nordeng H, Al-Zayadi W, Diallo D, Ballo N, Paulsen BS. Traditional medicine practitioners' knowledge and views on treatment of pregnant women in three regions of Mali. J Ethnobiol Ethnomed 2013;9:67. doi: 10.1186/1746-4269-9-67.
Macfoy C. Medicinal Plants and Traditional Medicine in Sierra Leone; 2013. Available from: iUniverse.com
. [Last Accessed on 13 Nov 2020].
Mothupi MC. Use of herbal medicine during pregnancy among women with access to public healthcare in Nairobi, Kenya: A cross-sectional survey. BMC Complement Altern Med 2014;14:432. doi: 10.1186/1472-6882-14-432.
Pieterse P, Lodge T. When free healthcare is not free. Corruption and mistrust in Sierra Leone's primary healthcare system immediately prior to the Ebola outbreak. Int Health 2015;7:400-4. doi: 10.1093/inthealth/ihv024.
James PB, Bah AJ, Kondorvoh IM. Exploring self-use, attitude and interest to study complementary and alternative medicine (CAM) among final year undergraduate medical, pharmacy and nursing students in Sierra Leone: A comparative study. BMC Complement Altern Med 2016;16:121. doi: 10.1186/s12906-016-1102-4.
James PB, Bah AJ. Awareness, use, attitude and perceived need for complementary and alternative medicine (CAM) education among undergraduate pharmacy students in Sierra Leone: A descriptive cross-sectional survey. BMC Complement Altern Med 2014;14:438. doi: 10.1186/1472-6882-14-438.
Witter S, Brikci N, Harris T, Williams R, Keen S, Mujica A, et al
. The Sierra Leone Free Health Care Initiative (FHCI): Process and Effectiveness Review; 2020. Available from https://core.ac.uk/download/pdf/42537333.pdf
. [Last retrieved on 2018 June 16].
Ahmed SM, Nordeng H, Sundby J, Aragaw YA, de Boer HJ. The use of medicinal plants by pregnant women in Africa: A systematic review. J Ethnopharmacol 2018;224:297-313. doi: 10.1016/j.jep.2018.05.032.
Berger E, Ashkenazi I. Jimson weed poisoning. Harefuah 2003;142:364-7, 397.
Nuhu H, Ghani A. Alkaloid content of the leaves of three Nigerian Datura
species. Niger J Nat Prod Med 2002;6:15-8. [doi: 10.4314/njnpm.v6i1.11685].
Oberndorfer S, Grisold W, Hinterholzer G, Rosner M. Coma with focal neurological signs caused by Datura stramonium
intoxication in a young man. J Neurol Neurosurg Psychiatry 2002;73:458-9. doi: 10.1136/jnnp.73.4.458-a.
Ruhwald M. Jimsonweed poisoning and the anticholinergic syndrome. Ugeskr Laeger 2005;167:2041-3.
Friedman M, Levin C. Composition of jimson weed (Datura stramonium
) seeds. J Agri Food Chem 1989;37:998-1005. [doi: 10.1021/jf00088a040].
Hussain F, Badshah L, Dastagir G. Folk medicinal uses of some plants of South Waziristan, Pakistan. Pak J Plant Sc 2006;12:27-39.
Egharevba R, Ikhatua M. Ethno-medical uses of plants in the treatment of various skin diseases in Ovia North East, Edo State, Nigeria. Res J Agri Biol Sci 2008;4:58-64.
Rajbhandari K. Ethnobotany of Nepal. Kathmandu: Kishor offset Press Private Limited; 2001.
Barrager E, Veltmann JR Jr., Schauss AG, Schiller RN. A multicentered, open-label trial on the safety and efficacy of methylsulfonylmethane in the treatment of seasonal allergic rhinitis. J Altern Complement Med 2002;8:167-73. doi: 10.1089/107555302317371451.
Mills E, Saunders P. Herbal Medicines in Pregnancy and Lactation. London: Taylor & Francis; 2006.
Djilani A, Legseir B, Soulimani R, Dicko A, Younos C. New extraction technique for alkaloids. J Braz Chem Soc 2006;17:518-20. [doi: 10.1590/s0103-50532006000300013].
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol 1983;54:275-87. doi: 10.1007/bf01234480.
Cora MC, Kooistra L, Travlos G. Vaginal cytology of the laboratory rat and mouse: Review and criteria for the staging of the estrous cycle using stained vaginal smears. Toxicol Pathol 2015;43:776-93. doi: 10.1177/0192623315570339.
Marcondes FK, Bianchi FJ, Tanno AP. Determination of the estrous cycle phases of rats: Some helpful considerations. Braz J Biol 2002;62:609-14. doi: 10.1590/s1519-69842002000400008.
Gaskin S, Tardif M, Cole E, Piterkin P, Kayello L, Mumby D. Object familiarization and novel-object preference in rats. Behav Processes 2010;83:61-71. [doi: 10.1016/j.beproc. 2009.10.003].
Ennaceur A, Delacour J. A new one-trial test for neurobiological studies of memory in rats. 1: Behavioral data. Behav Brain Res 1988;31:47-59. doi: 10.1016/0166-4328(88)90157-x.
Morris R. Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 1984;11:47-60. doi: 10.1016/0165-0270(84)90007-4.
Qing H, He G, Ly PT, Fox CJ, Staufenbiel M, Cai F, et al
. Valproic acid inhibits Abeta production, neuritic plaque formation, and behavioral deficits in Alzheimer's disease mouse models. J Exp Med 2008;205:2781-9. doi: 10.1084/jem.20081588.
Lillie R, Fullmer H. Histopathologic Technic and Practical Histochemistry. New York [USW], Düsseldorf: Mc-Graw-Hill; 1976.
Hanna JP, Schmidley JW, Braselton WE Jr. Datura
delirium. Clin Neuropharmacol 1992;15:109-13. doi: 10.1097/00002826-199204000-00004.
Ramirez M, Rivera E, Ereu C. Fifteen cases of atropine poisoning after honey ingestion. Vet Hum Toxicol 1999;41:19-20.
El Bazaoui A, Bellimam MA, Soulaymani A. Nine new tropane alkaloids from Datura stramonium
L. identified by GC/MS. Fitoterapia 2011;82:193-7. doi: 10.1016/j.fitote.2010.09.010.
Marín-Sáez J, Romero-González R, Garrido Frenich A. Enantiomeric determination and evaluation of the racemization process of atropine in Solanaceae
seeds and contaminated samples by high performance liquid chromatography-tandem mass spectrometry. J Chromatogr A 2016;1474:79-84. doi: 0.1016/j.chroma.2016.10.047.
VanPatten S, Al-Abed Y. The challenges of modulating the 'rest and digest' system: Acetylcholine receptors as drug targets. Drug Discov Today 2017;22:97-104. doi: 10.1016/j.drudis.2016.09.011.
Joëls M, Karst H, Alfarez D, Heine VM, Qin Y, van Riel E, et al
. Effects of chronic stress on structure and cell function in rat hippocampus and hypothalamus. Stress 2004;7:221-31. doi: 10.1080/10253890500070005.
Joosen MJ, Jousma E, van den Boom TM, Kuijpers WC, Smit AB, Lucassen PJ, et al
. Long-term cognitive deficits accompanied by reduced neurogenesis after soman poisoning. Neurotoxicology 2009;30:72-80. doi: 10.1016/j.neuro.2008.11.010.
Olawepo A, Ishola A, Ajao M, Olayemi O, Olayaki L. Atropine exposure in adolescence predisposes to adult memory loss in Wistar rats. Int J Biol Chem Sci 2018;11:1937. [doi: 10.4314/ijbcs.v11i5.1].
Alexander J, Benford D, Cockburn A, Cravedi J, Dogliotti E, Di Domenico A. Polycyclic aromatic hydrocarbons in food- Scientific Opinion of the panel on contaminants in the food chain. EFSA J 2008;6:724. [doi: 10.2903/j.efsa. 2008.724]. doi: 10.2903/j.efsa.2008.724.
Clewell RA, Gearhart JM. Pharmacokinetics of toxic chemicals in breast milk: Use of PBPK models to predict infant exposure. Environ Health Perspect 2002;110:A333-7. doi: 10.1289/ehp.021100333.
Hale TW. Anesthetic medications in breastfeeding mothers. J Hum Lact 1999;15:185-94. doi: 10.1177/089033449901500302
Káli S, Dayan P. Off-line replay maintains declarative memories in a model of hippocampal-neocortical interactions. Nat Neurosci 2004;7:286-94. doi: 10.1038/nn1202.
Koene RA, Gorchetchnikov A, Cannon RC, Hasselmo ME. Modelling goal-directed spatial navigation in the rat based on physiological data from the hippocampal formation. Neural Netw 2003;16:577-84. doi: 10.1016/s0893-6080(03)00106-0.
Bartsch T, Döhring J, Rohr A, Jansen O, Deuschl G. CA1 neurons in the human hippocampus are critical for autobiographical memory, mental time travel, and autonoetic consciousness. Proc Natl Acad Sci U S A 2011;108:17562-7. doi: 10.1073/pnas.1110266108.
Coyle JT, Puttfarcken P. Oxidative stress, glutamate, and neurodegenerative disorders. Science 1993;262:689-95. doi: 10.1126/science.7901908.
Lipton SA, Rosenberg PA. Excitatory amino acids as a final common pathway for neurologic disorders. N Engl J Med 1994;330:613-22. doi: 10.1056/nejm199403033300907.
Ankarcrona M, Dypbukt JM, Bonfoco E, Zhivotovsky B, Orrenius S, Lipton SA, et al
. Glutamate-induced neuronal death: A succession of necrosis or apoptosis depending on mitochondrial function. Neuron 1995;15:961-73. doi: 10.1016/0896-6273(95)90186-8.
Morrison R, Wenzel H, Kinoshita Y, Robbins C, Donehower L, Schwartzkroin P. Loss of the p53 tumour suppressor gene protects neurons from kainate-induced cell death. J Neurosci 1996;16:1337-45. [doi: 10.1523/jneurosci. 16-04-01337.1996].
Ekanem P, Ekanem R, Gaim K. (2016). Histological patterns of neurodegeneration of frontal cortex neurons in Datura stramonium
treated Wistar rats. J Behav Brain Sci 2016;6:85-92. [doi: 10.4236/jbbs. 2016.62009].
Bihaqi SW, Sharma M, Singh AP, Tiwari M. Neuroprotective role of Convolvulus pluricaulis
on aluminium induced neurotoxicity in rat brain. J Ethnopharmacol 2009;124:409-15. doi: 10.1016/j.jep.2009.05.038.
Bania TC, Chu J, Bailes D, O'Neill M. Jimson weed extract as a protective agent in severe organophosphate toxicity. Acad Emerg Med 2004;11:335-8. doi: 10.1197/j.aem.2003.12.002.
Hughes J, Clark J. Datura stramonium
poisoning. JAMA 1939;112:2500. [doi: 10.1001/jama. 1939.02800240016005].
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7]
|This article has been cited by|
||The prevalence, mechanism of action, and toxicity of Nigerian psychoactive plants
| ||Olamide Wilson Fasakin, Ganiyu Oboh, Ayokunle Olubode Ademosun |
| ||Comparative Clinical Pathology. 2022; |
|[Pubmed] | [DOI]|
||The modulatory effects of alkaloid extracts of Cannabis sativa, Datura stramonium, Nicotiana tabacum and male Carica papaya on neurotransmitter, neurotrophic and neuroinflammatory systems linked to anxiety and depression
| ||Olamide Wilson Fasakin, Ganiyu Oboh, Ayokunle Olubode Ademosun, Akeem O. Lawal |
| ||Inflammopharmacology. 2022; |
|[Pubmed] | [DOI]|